Developed in a laboratory, a monoclonal antibody (mAb) is designed to attach to a certain type of antigen – for example, to the spike protein of the coronavirus or to particular markers on cancer cells. As such, mAbs are used as a targeted treatment for different medical conditions as they support the body’s immune system. At Cell Culture Company, we are your source for monoclonal antibody production and analysis.
Monoclonal antibodies have become vital in modern medicine. Clinical trials are performed in the search for new ways to use mAbs for the advancement of public health . The awareness of monoclonal antibodies and their impact on public health has been heightened in the public as a result of the COVID-19 pandemic.
Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay technique developed for the detection and quantification of various soluble substances, such as antibodies, proteins, peptides, and hormones. The same technology is referred to by enzyme immunoassay (EIA) and other names.
With an ELISA, the target macromolecule – the antigen – is attached to a microplate (solid surface) and then made to form a complex with an antibody connected to a reporter enzyme. Incubation with the proper substrate is used to measure the activity of the reporter enzyme. This enables detection and the production of a measurable product. A highly specific antigen-antibody interaction is the most vital element of an ELISA.
ELISAs in Diagnostics
Therapies rely on diagnostics using monoclonal antibodies to inform proper treatments of various diseases. At the same time, treatments can pose some risks, making accurate and reliable reagents essential to diagnostic tests. This is why it is important to partner with a contract development and manufacturing organization that offers expertise and accuracy in antibody analysis and characterization.
At Cell Culture Company, we provide monoclonal antibody testing and analysis under strict quality control conditions. The testing we employ helps ensure optimum reproducibility and yield before the start of large-scale biomanufacturing.
For information about our monoclonal antibody production services, call us today at 763.786.0302 or send us a message through our contact form.