Cell Line Characterization Services

Ensure the integrity and reliability of your research with our comprehensive cell line characterization services. Accurate cell line characterization is critical for validating experimental results, maintaining consistency, and advancing scientific discoveries. Our scientific staff and expert support provide a thorough analysis of your cell lines, offering detailed insights into their genetic, phenotypic, and functional properties.

Our Cell Line Characterization Services Include:

1. Genetic Authentication:

  • Short Tandem Repeat (STR) Profiling: Confirm cell line identity by comparing unique genetic fingerprints against reference databases.
  • Karyotyping and Chromosomal Analysis: Detect chromosomal abnormalities and genetic stability to ensure cell line authenticity.
  • Cell Line Purity Confirmation:  Confirm your cultures are pure and free from unwanted contaminants, including other cell lines.
  • Next Generation Sequencing (NGS): Sequence entire genomes, transcriptomes, or targeted regions of interest with unprecedented speed
  • Comparative Genomic Hybridization (CGH): A molecular cytogenetic technique used to detect chromosomal copy number variations (CNVs) across an entire genome.

2. Gene Expression Analysis:

  • Quantitative PCR (RT-qPCR / qPCR): Accurately quantify gene expression levels, providing insights into cellular functions and regulatory mechanisms.
  • RNA Sequencing (RNA-Seq): Comprehensive transcriptome analysis to explore gene expression profiles and discover novel transcripts.
  • siRNA knockdown: Confirm the functionality of a cell to silence a gene of interest.
  • Fluorescence In Situ Hybridization (FISH):  Detect and localize the presence or absence of specific DNA sequences within chromosomes or RNA within cells and tissues.

3. Protein Analysis:

  • Western Blotting: Detect and quantify specific proteins to assess expression levels, post-translational modifications, and protein-protein interactions.
  • Isoenzyme Analysis: Isoenzyme analysis is a valuable technique in biochemical and biomedical research, offering insights into enzyme diversity, genetic variation, and disease biomarkers.

4. Phenotypic Characterization:

  • Flow Cytometry: Analyze cell surface markers, intracellular proteins, and cell cycle status to determine cell phenotype and functional properties.
  • Immunocytochemistry (ICC): Visualize protein localization and expression within cells using fluorescent or chromogenic staining techniques.
  • Immunohistochemistry (IHC):  using antibodies labeled with a chromogen or fluorophore that bind to antigens within tissue sections.

5. Functional Assays:

  • Cell Proliferation and Viability: Assess cell growth rates and viability under various conditions to evaluate cell health and response to treatments.
  • Functional Transporters:  Confirm that transporters are present and functional.
  • Proper expression: Challenge the cell line with a known stimulus to confirm the expected appropriate response.

Elevate your research with our comprehensive cell line characterization services. Ensure the authenticity, stability, and functionality of your cell lines to drive scientific breakthroughs and maintain the highest standards of research integrity. Contact us today to learn more about how our expertise and technology can support your cell line characterization needs.

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