Perfusion cell culture is a cell culture method where cells are grown in a perfusion system. This type of cell culture is often used for large-scale production of cells or for cells that require a constant supply of fresh media.
Perfusion cell culture systems offer many advantages over static cell culture systems, including:
- Increased cell yields
- Reduced chance of contamination
- Reduced risk of media degradation
- More consistent cell growth and product quality
Biopharma companies increasingly use perfusion cell culture systems to produce therapeutic proteins and monoclonal antibodies. In addition, they are being used in regenerative medicine to grow cells for tissue engineering and cell therapy applications.
Perfusion cell culture systems typically consist of a perfusion device, a sterile supply of media, and a culture vessel. The perfusion device circulates the media through the culture vessel, providing fresh media to the cells and removing waste products.
Cells are seeded into the culture vessel and allowed to proliferate. Once the cells have reached a specific density, the perfusion system is turned on. The media is circulated through the culture vessel.
While static cell culture systems usually have a low entry cost, they tend to cost more long term compared to perfusion cell culture systems due to the advantages listed above.
If you are interested in perfusion cell culture, Cell Culture Company can help you get started. With over 40 years of experience in cell culture, our team can provide you with the expertise and products you need to succeed. Contact us today to learn more.