Monoclonal Antibody Screening and Production

Monoclonal antibodies (mAbs) stem from a single, unique parent cell. As a result, these antibodies only bind to a single epitope. The production of monoclonal antibodies is crucial to research and treatment efforts regarding various diseases, both infectious and non-infectious. At Cell Culture Company, we provide customized production of monoclonal antibodies. Our team has manufactured hundreds of client monoclonal antibodies over our 40 years in business. We have the know-how and capability to carry out mass-scale antibody production.

Monoclonal Antibody Screening

The discovery process connected with monoclonal antibodies refers to the screening and identification of certain antibodies that pinpoint a specific epitope (part of an antigen molecule) for the diagnosis and treatment of various ailments – a recent example being COVID-19.

Monoclonal Antibody Production

The treatment of cancer has been enhanced recently by the design and production of monoclonal antibodies. The monoclonal antibodies we produce at Cell Culture Company have been utilized as reagents for diagnostics, research, and the development of products.

The production of custom monoclonal antibodies is a technical, time-consuming, and demanding task. Customers may give our team their antibody-producing hybridoma or other cell lines. Our scientists are able to provide monoclonal antibodies customized to the specifications of the customer.

The generation of monoclonal antibodies often involves the immunization of mice, generating hybridoma, clone screening, and ramping up the production of clones. At Cell Culture Company, we offer an alternative process using perfusion-based bioreactors to produce monoclonal antibodies that are easy-to-scale.

The process begins with inoculating a single-use hollow fiber cartridge by which a continual flow of media transports nutrients throughout the semi-permeable fibers. For the purposes of antibody production, the bioreactor environment referred to here is monitored and sustained at ideal conditions. The bioreactor is responsible for a concentrated and continual stream of harvested product over a period of several weeks to months.

At Cell Culture Company, we offer an array of services, including downstream processing and purification of monoclonal antibodies. To learn about our specialized process for the production of antibodies, give us a call today at 763.786.0302 or reach us through our contact form.

An Introduction to Mammalian Cell Culture

Mammalian cell culture is a highly regarded technique with academic and research applications. It is a requisite skill for researchers whose work requires consistent, scaleable quantities of human and animal cells for pharmaceutical, immunology, toxicological, and molecular biology research. If you are interested in learning about mammalian cell culture and its vast range of applications, here’s a brief look into its study.

What is mammalian cell culture?

This refers to the process of growing cells taken from mammals in-vitro. That is, growing cells outside the original tissue in a growth medium with the right nutrients. Mammalian cells are eukaryotic, meaning the individual cells are more complex than bacterial cells, which are often used to teach simple cell culturing techniques.

This complexity also stems from the specialization of the different cells in the various organs and tissues. Mammalian cells are grouped into cell types depending on their origin, function and morphology.

Morphology of a cell determines how it is grown in cell culture. Most cells can be grouped by one of two growth conditions – adherent or suspension.

  • Adherent cells are grown on a flat surface where they adhere and grow to occupy the surface they are seeded upon.
  • Suspension cells are free-floating in solution and occupy the entire three-dimensional volume of the culture vessel.

Matching a cell’s growth condition to a culture vessel is critical to proper and effective mammalian cell culture.

Uses for mammalian cell culture

Understanding cell biology is the key driving factor of all biotechnical advancements, especially in research and medicine. Damage can occur to any of the body cells as a result of disease and injury. A deep understanding of cell biology and cell types allows researchers and scientists to develop appropriate treatment methods.

Additionally, research into mammalian cell structure can be utilized in the following ways.

  • Production of biological products such as antibodies, enzymes, hormones, etc. Recombinant antibody expression combines cutting edge molecular biology techniques with mammalian cell culture to create antibody products superior in purity and scalability to those produced using traditional methods. Using cell culture for the controlled production of biological molecules has opened up new opportunities for research and medicine that were once much less accessible.
  • Manufacture of vaccines to protect people from viral and bacterial diseases. One of the most important research models in any biological field and medicine is the in-vitro study of mammalian cells. Mammalian cell culture has been used for the development and manufacture of vaccines, which has a massive positive impact on public health. Even though it requires specialized tools and rigorous antiseptic measures to ensure no cross-contamination occurs, the proven benefits and potential promise of their application are boundless.