Mammalian Cell Culture Without Ascites
Mammalian cell culture involves the growth of cells in a medium which is external to their tissue of origin. It is a useful tool for pharmaceutical, clinical, and research applications. When cells are isolated from their original animal tissues, they can be expanded and studied in culture in relation to cell biology and disease, or for the development of proteins, antibodies, and vaccines. The production of immortalized mammalian cell lines in- vitro is possible for extended periods of time. These cell lines are often used as basic models for more complex biology. At Cell Culture Company, we understand the various ways in which mammalian cell culture with and without ascites may be used.
From “With Ascites” to “Without Ascites” (In Vitro)
There are two common methods used in the production of quality monoclonal antibodies – in- vivo and in-vitro. In-vivo production, which uses ascites, is able to generate high concentrations of high-affinity monoclonal antibodies. It is also able to facilitate glycosylation and other post-translational changes within the animal model. The in-vivo method has enabled the development of thousands of various antibodies for therapeutic use and research. Some of the limiting challenges with ascites involve ethical concerns pertaining to the mouse models used, and facility logistical challenges with raising live animals Due to these concerns, in-vitro methods have become the preferred model for the production of antibodies.
Mammalian Cell Growth In Vitro
The in-vitro method of mammalian cell growth, such as in our hollow fiber bioreactors, makes a way around the ethical concerns associated with animal use through the growth of hybridoma cells in culture media. With this method, the cells dispense antibodies directly into the culture medium. The result of this approach is the production of quality antibodies at large-scale production and easy purification.
In order for cells to grow, they need a complex combination of nutrients, including vitamins, amino acids, minerals, sugars, albumin, and growth factors. The supply of these essential nutrients for in-vitro mammalian cell culture comes from cell culture media. Incubators are used to provide a sterile environment for growth with a temperature and CO2 concentration that provide a pH level close to that of mammalian blood.
The majority of cells stemming from mammalian tissues necessitate adherence to a surface for the proper division and growth of cells., Surfaces for cell cultures are available through treated polystyrene or glass flasks, filters, and plates designed and manufactured for cell attachment. Synthetic polymers, collagen, poly-D-lysine (PDL) or other extracellular matrices, include an attachment structure enabling adhesion.