Why Mycoplasma Testing Is Critical for Mammalian Cell Cultures
Introduction
Mycoplasma contamination is one of the most persistent and problematic threats in mammalian cell culture. These tiny, bacteria can silently infiltrate cultures, distorting experimental results and compromising the integrity of cell culture production. Despite their size, their impact is enormous ranging from altered cell growth to invalidated data and failed therapeutics. Keep reading to learn why mycoplasma testing for mammalian cell cultures is essential.
What Is Mycoplasma?
Mycoplasma are among the smallest known bacteria, lacking a rigid cell wall. This structural simplicity enables them to pass through standard filters and evade detection under typical microscopic examination. Common sources of contamination include contaminated media, infected lab personnel, and cross-contaminated cell lines.
Their stealth makes them a significant biosecurity concern, particularly in labs working with mammalian cells for diagnostics, biologics production, and research.
Why Mycoplasma Testing Matters
Mycoplasma contamination doesn’t always lead to immediate visible effects, which is why routine testing is essential. Over time, contamination can:
- Suppress cell growth and alter metabolism
- Skew gene expression and protein production
- Compromise reproducibility and regulatory compliance
- Lead to substantial financial losses in biotech workflows
Routine testing ensures scientific integrity and helps protect valuable research and commercial production efforts.
Methods for Detection
Several testing methods are available, including:
- PCR-based detection: Fast and highly sensitive, useful for screening multiple lines.
- Culture-based assays: Allow viable mycoplasma to grow in specialized media, providing definitive confirmation.
- DNA staining: Uses fluorochrome dyes to identify characteristic mycoplasma DNA in contaminated cells.
The most reliable labs implement a combination of these tests to ensure accuracy.
Preventing Contamination
Prevention starts with good aseptic technique, quarantining new cell lines, and regular testing. Working with a trusted partner for cell banking and screening, like Cell Culture Company, ensures that your cell lines are not only mycoplasma-free but also consistently characterized and ready for regulated applications.
Partner With Experts in Cell Quality
At Cell Culture Company, we offer comprehensive mycoplasma testing as part of our cell line development, banking, and production services. Our clients count on us to deliver validated, contamination-free cell lines that meet the highest standards in research and manufacturing. Contact us to discuss your needs.
