Monoclonal Antibody Screening and Production

Monoclonal antibodies (mAbs) are generated from a single parent cell, which allows them to bind to one specific epitope. This high specificity makes mAbs essential tools in both diagnostics and therapeutics. At Cell Culture Company, we specialize in custom monoclonal antibody production. With over 40 years of experience, we’ve manufactured hundreds of antibodies from our clients’ cell lines and are equipped to handle small- and large-scale production.

Precision Screening for Specific Epitopes

Monoclonal antibody development begins with the identification and screening of antibodies that recognize a particular epitope—a unique region on an antigen. These antibodies have contributed significantly to diagnosing and treating a wide range of diseases. For instance, during the COVID-19 pandemic, monoclonal antibodies were a treatment approach.

Accurate screening ensures that the resulting antibodies exhibit the desired specificity and affinity. This phase is vital for ensuring reproducibility and reliability in applications such as research, diagnostics, or product development.

Custom Antibody Production for Research and Diagnostics

Monoclonal antibodies have transformed cancer immunotherapy by targeting tumor-specific antigens (via cancer.gov). Beyond this, they are widely used as reagents in research and diagnostic assays. At Cell Culture Company, we produce high quality monoclonal antibodies from customer-provided hybridomas or other cell lines.

Our team works closely with clients to meet unique specifications for concentration, isotype, and buffer conditions. Although custom antibody generation can be complex, our expertise simplifies the process.

Streamlined Bioreactor-Based Production

Traditional methods involve immunizing mice, generating hybridomas, and scaling up production through multiple steps. At Cell Culture Company, we offer a more efficient alternative using perfusion-based hollow fiber bioreactors.

This process begins with inoculating a single-use hollow fiber cartridge. Nutrient-rich media continuously flows through semi-permeable fibers, providing optimal growth conditions. The bioreactor maintains a stable environment, allowing continuous harvesting over weeks or even months.

Key Advantages of Hollow Fiber Bioreactors:

Continuous product harvesting over extended periods
High-density culture for increased antibody yield
Scalable platform ideal for research or pre-commercial needs
Minimal batch-to-batch variability
Reduced contamination risk with closed-system operation

As a result, our clients benefit from consistent output with less intervention and greater efficiency.

Complete Antibody Purification Services

We don’t stop at production. Our scientists also provide processing and purification services to deliver functional, high-purity monoclonal antibodies. Whether you’re advancing a diagnostic assay or scaling up a reagent supply, we deliver solutions to meet your goals.

For more background on antibody applications in biotechnology, refer to Nature’s Biotechnology journal (nature.com) or PubMed on ncbi.nlm.nih.gov.

Partner With Cell Culture Company

With decades of experience and state-of-the-art technology, Cell Culture Company is your trusted partner in monoclonal antibody production. From early-stage screening to scalable production and purification, we offer a complete suite of services designed for reliability and performance.