Cell Line Development Services

Our Services Include:

Cell Line Development Services Fit for Purpose: Creation of appropriate cell lines for the production of proteins, antibodies, functional transporters, and more.
Custom Gene Editing: CRISPR/Cas9 and other gene-editing technologies to create cell lines with specific genetic modifications.
Stable Cell Line Generation: Development of cell lines with stable expression of your gene of interest for long-term studies and production.
Transient Expression Systems: Rapid production of recombinant proteins and antibodies using transient expression techniques.

Genetically Tailored Cellular Solutions

Genetically engineered cell lines are widely used in various areas, including recombinant protein production, in vitro modeling, functional analysis and R&D.
With experience in protein/antibody production, scientists at Cell Culture Company have established a complete process solution for cell line development.
We tailor to your production needs. With increase productivity and stability, integrating all experimental steps, we provide a one-stop service from gene sequence to cell line development, ready-to-use, and recombinant protein production.

Engineering Services for Cell Line Development: Process

Consultation and Planning: Collaborate with our experts to define your project goals and requirements.
Design and Construction: We help you to select all necessary constructs and delivery systems to your chosen cell line.
Selection and Screening: We identify and isolate the highest-performing clones through rigorous testing and analysis.
Characterization and Validation: Thoroughly characterize the selected clones for stability, productivity, and desired traits.
Delivery and Support: Deliver cell lines along with comprehensive characterization data and follow up with your downstream processes and success of your project.

Stable Cell Line – Service Workflow

Gene Synthesis

We can make our constructs to over-express any genes (wildtype, mutant or synthetic) and to silence/knockout any genes from any species if you don’t have any starting material. Our scientists are available to discuss your specific requirement and come up with the recommended constructs for your specific needed. We can then produce the final construct based on the agreed specification and full-length validated by Next Generation Sequencing as QC.

Construct Delivery

All clonal stable cell lines are developed based on individual client specifications, using highly efficient gene delivery technologies to produce clonal cell lines. We can produce multiple clonal cell lines depending on your needs, with various expression levels (low / medium / high expression), reprogrammed and genetically modified. Typically, antibiotic resistance or fluorescent reporter gene markers are incorporated into the plasmid DNA construct to facilitate selection process. These selection markers can be co-expressed on the same vector or independently. expressed.

Transfection Analysis

Standard services include transfection of plasmid DNA and/or whole complex CRISPR/Cas9 RNPs, drug selection of clonal cells, colony pick up, generation of a stable line, expression and functional screening, and final validation of construct expression by qRT-PCR and/or Western blot analysis.

Culturing and Selection

Clone Screening

After we have determined the cleavage efficiency of the pooled cell population, isolate single cell clones for further validation and banking. We isolate single cell clones from the selected pool using limiting dilution cloning (LDC) in 96‑well plates.

From single-cell cloning and colony outgrowth to the establishment of a master cell bank, our portfolio of technologies and cell growth supplements and matrices deliver accelerated workflows while confidently ensuring clonality.

Verification Post-Isolation

We apply a variety of techniques to characterize genetically engineered cell lines to physiologically and molecularly evaluate the modification in the final cell line.

Techniques such as: Immunofluorescence for a qualitative analysis and flow cytometry of quantitative population within the final cell lines.

Moreover, the genomic integration of the target gene and associated mRNA expression increase can be verified using quantitative real-time RT-PCR. The Western blot assay uses antibodies specific to the target protein in order to quantify protein in a sample. The utilization of both techniques validates that the target gene is both integrated into the genome and functionally expressing.

Final Production and Delivery

We create a master cell bank with at least 10 passages of 3 different clones. We send vials of each passage and keep vials safe in the bank for an extra banking charge in case you need extra vials for security. The shipment will include a cell pool/ clone generation report and we will follow up with any step of you downstream processes to ensure the success of your project.

Why Choose Our Cell Line Development Services?

Customized Solutions: Understanding that each project is unique, we offer tailored solutions to fit your specific requirements. From initial consultation to final delivery, we work closely with you to ensure your research and production goals.
Advanced Technologies: We leverage the latest technologies in cell line development to provide you with the most efficient and effective solutions.
Rigorous Quality Control: Quality is at the core of our services. We employ stringent quality control measures throughout the process ensuring the stability, productivity, and consistency of your cell lines. Our robust testing protocols include genetic stability assessments, growth rate analysis, and protein expression validation.
Fast Turnaround Times: Our streamlined processes and efficient workflows are designed to deliver high-quality cell lines in the shortest possible time.
Comprehensive Support: From project inception to completion to continued post project support, our dedicated scientific team is here to assist you. We provide weekly progress reports, technical support, and troubleshooting to ensure the success of your project.

Vision

Vision→ To offer not only a cell line engineering and development service but also streamline processes.
Goal→ End to failed product development through our experienced strategic solutions and experimentation with any processes involving cell characterization, modification and protein
- From proof of concept to scalability and manufacturing.
- Testing and validate any cell-based model for mechanisms of action, potency and toxicity of products (cosmetic, agriculture, pharma and biopharma).

Partner with Cell Culture Company for your cell line development needs to accelerate your research with confidence. Contact us today to learn more and how we help you achieve your critical research and production needs.